Feugiat nulla facilisis at vero eros et curt accumsan et iusto odio dignissim qui blandit praesent luptatum zzril.
+ (123) 1800-453-1546

Related Posts

Neutrophils require SKAP2 for reactive oxygen species production following C-type lectin and Candida stimulation

July 16, 2021

Giang T Nguyen, Shuying Xu, Walter Adams, John M Leong, Stephen C Bunnell, Michael K Mansour, David B Sykes, Joan Mecsas

iScience. 2021 Jul 16;24(8):102871. eCollection 2021 Aug 20.
PMID: 34386732 | PMCID: PMC8346660 | DOI: 10.1016/j.isci.2021.102871


Signaling cascades converting the recognition of pathogens to efficient inflammatory responses by neutrophils are critical for host survival. SKAP2, an adaptor protein, is required for reactive oxygen species (ROS) generation following neutrophil stimulation by integrins, formyl peptide receptors, and for host defense against the Gram-negative bacterial pathogens, Klebsiella pneumoniae and Yersinia pseudotuberculosis. Using neutrophils from murine HoxB8-immortalized progenitors, we show that SKAP2 in neutrophils is crucial for maximal ROS response to purified C-type lectin receptor agonists and to the fungal pathogens, Candida glabrata and Candida albicans, and for robust killing of C. glabrata. Inside-out signaling to integrin and Syk phosphorylation occurred independently of SKAP2 after Candida infection. However, Pyk2, ERK1/2, and p38 phosphorylation were significantly reduced after infection with C. glabrata and K. pneumoniae in Skap2-/-neutrophils. These data demonstrate the importance of SKAP2 in ROS generation and host defense beyond antibacterial immunity to include CLRs and Candida species.


Source: https://pubmed.ncbi.nlm.nih.gov/34386732/